Whole Foods faces four charges under the Ontario Retail Business Holiday Act for opening its Lansdowne Park store on Good Friday.
The charges against the high-end grocery store were laid by Ottawa Police following a complaint launched by a resident.
The fine for a first offence under provincial law is $500.
Fines for repeat offences can reach up to $50 thousand.
Whole Foods states it opened its doors on Good Friday under the premise Lansdowne Park was a tourist area exempt under the retail holiday law.
In Ottawa, the exemptions granted by City Council only appy to the Byward Market area and Sparks Street.
A court date has been scheduled for May 21, 2015.